Appeal No. 2001-2401
Application 08/277,225
(b) limiting the contact time between said second ligand and said solid phase
so that:
(i) non-specific binding between said second ligand and said solid
phase is not allowed to reach equilibrium; and
(ii) a first binding curve, in which formation of a non-specific second
ligand/solid phase complex is plotted versus time, does not level off;
(c) binding a detectable tag to said second ligand either prior to or after
formation of said first ligand/analyte/second ligand complex so that a portion of said tag
is retained on said solid phase upon formation of said first ligand/analyte/second ligand
complex;
(d) detecting said retained tag to determine the presence or level of said
analyte in said sample.
23. A method of detecting the presence or level of an analyte in a sample, the
method comprising steps of:
(a) contacting said sample with:
(i) a solid phase having bound thereto a first ligand, which first
ligand is characterized in that it binds to said analyte when incubated therewith, the
contacting being performed so that a first ligand/analyte complex is formed on said solid
phase; and
(ii) a second ligand that binds said first ligand/analyte/second ligand
complex when incubated therewith, so that a first ligand/analyte/second ligand complex
is formed on said solid phase, said contacting being performed under contacting being
performed under conditions and for a time sufficiently limited that substantially no non-
specific binding between said second ligand and said solid phase occurs;
(b) binding a detectable tag to said second ligand either prior to or
after formation of said first ligand/analyte/second ligand complex so that a portion of
said tag is retained on said solid phase upon formation of said first
ligand/analyte/second ligand complex;
(c) detecting said retained tag to determine the presence or level of
said analyte in said sample.
The references relied upon by the examiner are:
3
Page: Previous 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Next
Last modified: November 3, 2007