(b) the replacement of a nonessential portion of said replicable
vesiculovirus genomic sense (-) RNA with a foreign or
heterologous RNA sequence;
in which an RNA sequence complementary to said foreign RNA sequence
encodes a peptide or protein. [Rose claim 75, as amended)
OR
A modified inactivated recombinant vesiculovirus that is the produce of a
method comprising inactivating a recombinant replicable vesiculovirus, said
recombinant replicable vesiculovirus comprising N, P, and L proteins, and a
replicable vesiculovirus genomic sense (-) RNA, in which said genomic sense
RNA, is modified by:
(c) the insertion of a foreign or heterologous RNA sequence
into a nonessential portion of said replicable vesiculovirus
genomic sense (-) RNA; or
(d) the replacement of a nonessential portion of said replicable
vesiculovirus genomic sense (-) RNA with a foreign or
heterologous RNA sequence;
in which an RNA sequence complementary to said foreign RNA sequence
encodes a peptide or protein. [Rose claim 103, as amended]
OR
A pure, recombinant, replicating and infectious vesicular stornatitus virus
(VSV) particle, comprising:
1) a functional RNA dependent RNA polymerase (L);
2) a vesiculovirus phosphoprotein (P);
3) a vesiculovinis nucleocapsid (N);
4) vesiculovirus protein selected from the group consisting of
glycoprotein (G) and matrix (M)
5) a Ynon-coding RNA sequence;
6) a Yto 5'RNA coding sequence, which encodes the
vesiculovirus L, P, N, and vesiculovirus protein required
for assembly of budded infectious particles and includes a
heterologous gene (X), wh-.rein said heterologous gene (X)
is inserted at an intergenic junction; and
7) a 5'non-coding RNA sequence, and wherein components I
through 7 are from the same type of VSV. [Rose claim 132,
unamended], and
7
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Last modified: November 3, 2007