Appeal No. 2003-1783
Application No. 09/560,472
5. A method for reducing non-specific adsorption of inorganic ions, peptides,
proteins, and saccharides to a surface of a device comprising
applying to or coating onto a surface of an analytical or sensing device, wherein
the surface comprises a material selected from the group consisting of metals, metal
oxides, and charged polymers,
a non-interactive polyionic multifunctional copolymer, wherein the non-interactive
polyionic multifunctional copolymer comprises non-interactive polymer sidechains
covalently grafted onto a charged polyionic polymeric backbone which has an anionic
charge at a pH greater than 4, wherein the polyionic polymeric backbone interacts with
the surface at a pH greater than 4.
13. The method of claim 9 [the method of claim 5 where some or all of the non-
interactive polymer sidechains are partially or fully functionalized at or near the free
terminal position of the non-interactive polymer sidechains with a functional molecule.]
comprising exposing the copolymer to analyte which is bound by the functional
molecule in a fluid phase, followed by adsorption of the copolymer onto the substrate or
surface.
15. A method for making a biosensing or analytical device having reduced non-
specific adsorption of inorganic ions, peptides, proteins, and saccharides comprising
applying to or coating onto a surface of the biosensing or analytical device non-
interactive polyionic multifunctional copolymers,
wherein the non-interactive polyionic multifunctional copolymers comprise non-
interactive polymer sidechains covalently grafted onto a charged polyionic polymeric
backbone, and
wherein the surface comprises a material selected from the group consisting of
metals, metal oxides, and charged polymers, and
the surface of the device has a charge opposite to the charge of the charged
polyionic polymeric backbone, and
wherein some of the non-interactive polymer side chains comprise a functional
moiety and some of the non-interactive polymer side chains do not comprise a
functional moiety.
20. The method of claim 15 wherein the surface is patterned with areas with
adsorbed polyionic copolymers not comprising functional groups and areas with
adsorbed polyionic copolymers comprising functional groups.
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