Appeal No. 2003-1918
Application No. 08/945,722
APPENDIX
1. A starch comprising granules extracted from a potato
plant, said starch having an amylose content of at least 35%, as
judged by the iodometric assay method of Morrison & Laignelet.
64. A starch obtainable from a plant having characteristics
altered by a method selected from the group consisting of
(a) introducing into the plant a portion of a nucleotide
sequence encoding an effective portion of a class A starch
branching enzyme (SBE) obtainable from potato plants to
complement the branching enzyme mutation in E coil KV 832 cells
and which is active when expressed in E. coli in the
phosphorylation stimulation assay operably linked to a suitable
promoter active in the plant so as to affect the expression of a
gene present in the plant;
(b) introducing into the plant a portion of a nucleotide
sequence encoding an effective portion of a class A starch
branching enzyme (SBE) obtainable from potato plants operably
linked to a suitable promoter active in the plant so as to affect
the expression of a gene present in the plant, wherein the
nucleotide sequence is operably lined in the anti-sense
orientation to a suitable promoter active in the plant;
(c) introducing into the plant a portion of a nucleotide
sequence encoding an effective portion of a class A starch
branching enzyme (SBE) obtainable from potato plants operably
linked to a suitable promoter active in the plant so as to affect
the expression of a gene present in the plant, wherein the
introduced sequence comprises at least one region selected from
the group consisting of a 5' untranslated region, a 3'
untranslated region, and a coding region of the potato SBE class
A SBE operably linked in the sense orientation to a promoter
active in the plant;
(d) introducing into the plant a portion of a nucleotide
sequence encoding an effective portion of a class A starch
branching enzyme (SBE) obtainable from potato plants operably
linked to a suitable promoter active in the plant so as to affect
the expression of a gene present in the plant further comprising
introducing into the plant one or more further sequences;
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