Appeal No. 2003-1918
Application No. 08/945,722
(e) introducing into the plant a portion of a nucleotide
sequence encoding an effective portion of a class A starch
branching enzyme (SBE) obtainable from potato plants operably
linked to a suitable promoter active in the plant so as to affect
the expression of a gene present in the plant further comprising
introducing into the plant one or more further sequences operably
linked in the anti-sense orientation to a suitable promoter
active in the plant; and
(f) introducing into the plant a portion of a nucleotide
sequence encoding an effective portion of a class A starch
branching enzyme (SBE) obtainable from potato plants operably
linked to a suitable promoter active in the plant so as to affect
the expression of a gene present in the plant further comprising
introducing into the plant a portion of a class B SBE nucleotide
sequence;
wherein the portion is effective to complement the branching
enzyme mutation in E coli KV 832 cells and which is active when
expressed in E. coli in the phosphorylation stimulation assay,
and the starch has an amylose content of at least 35% as judged
by the iodometric assay method of Morrison & Laignelet.
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