Appeal No. 2004-2134 Page 2
Application No. 09/425,075
said second expression cassette comprises a second promoter operably
linked to a nucleic acid encoding an immunoglobulin heavy chain operably linked
to a second signal peptide,
and said culturing provides for expression of the immunoglobulin light and
heavy chains; and
harvesting specific antigen-binding antibody from culture supernatant,
which antibody specifically binds an antigen of interest.
41. The method of Claim 36, wherein said antibody specifically binds dioxin.
The examiner relies upon the following references:
Robinson et al. (Robinson) 6,204,023 Mar. 20, 2001
Vanderlaan et al. (Vanderlaan) 5,429,925 Jul. 04, 1995
Horwitz et al. (Horwitz) “Secretion of Functional Antibody and Fab Fragment from
Yeast Cells,” Proc. Natl. Acad. Sci. USA, Vol. 85, pp.8678-8682 (1988)
Cregg et al. (Cregg) “Development of the Methylotrophic Yeast, Pichia Pastoris,
as a host System for the Production of Foreign Proteins,” Developments in
Industrial Microbiology, Vol. 29, pp.33-41 (1988)
The Invitrogen Catalog “Yeast Expression,” pp. 14-19 (1997)
In addition, appellants rely upon the following references:
Holliger “Expression of Antibody Fragments in Pichia pastoris,” Methods in
Molecular Biology, Vol. 178, pp. 348-357 (2002)
Pennell et al. (Pennell) “In vitro Production of Recombinant Antibody Fragments
in Pichia pastoris,” Res Immunol, Vol. 149, pp. 599-603 (1998)
Page: Previous 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Next
Last modified: November 3, 2007