Appeal No. 2005-0394 Page 2
Application No. 08/940,692
(c) having a specific growth rate on glucose as a sole carbon
source of at least 0.4h-1.
27. A method for increasing PEP availability to a biosynthetic or metabolic
pathway of a host cell, the method comprising,
a) obtaining a host cell mutant characterized by having a Pts-/glu+
phenotype requiring galactose permease activity to transport
glucose; and having a specific growth rate on glucose as a sole
carbon source of at least 0.4h-1 wherein the Pts- phenotype is
caused by the deletion or inactivation of all or substantially all of
one of the genes selected from the group consisting of ptsI,
ptsH and crr; and
b) culturing the host cell mutant in the presence of an appropriate
carbon source, wherein said host cell mutant utilizes PEP as a
precursor or intermediate of metabolism.
38. A method for obtaining a Pts-/Glucose+, glactose permease requiring-
mutant cell, the method comprising:
(a) selecting a host cell which utilizes a phosphotransferase
transport system;
(b) mutating the host cell whereby the phosphotransferase
transport system is inactivated;
(c) culturing the mutant host cell under continuous culture
conditions using glucose as a carbon source; and
(d) selecting mutant host cells which grow on glucose at a specific
growth rate of at least 0.4h-1
The references relied upon by the examiner are:
Frost 5,168,056 Dec. 1, 1992
Ingrahm et al. (Ingrahm) 5,602,030 Feb. 11, 1997
Holms, “The Central Metabolic Pathways of Escherichia coli: Relationship
Between Flux and Control Branch Point, Efficiency of Conversion to Biomass,
and Excretion of Acetate,” Curr. Topics Cell. Regulation, Vol. 28, pp. 66-105
(1986)
Saier et al. (Saier), “characterization of Constitutive Galactose Permease
Mutants in Salmonella typhirmurium,” J. Bacteriol, Vol. 113, No. 1, pp. 512-514
(1991)
GROUNDS OF REJECTION
Claims 23, 27, 38, 46 and 49 stand rejected under 35 U.S.C. § 103 as
being unpatentable over Saier and Ingrahm.
Page: Previous 1 2 3 4 5 6 7 8 9 Next
Last modified: November 3, 2007