Appeal No. 2006-2851
Application No. 09/844,501
(d) contacting the deproteinized DNA with a second enzyme to generate DNA
fragments;
(e) contacting the DNA fragments obtained in step (d) with a population of vector
molecules, wherein the vector molecules comprise a first end that is compatible with the
first enzyme and a second end that is compatible with the second enzyme, under
conditions favorable to ligation of compatible ends; and
(f) selecting polynucleotides comprising a DNA fragment ligated to a vector
molecule.
The prior art cited by the examiner is:
Li et al. (Li) 5,500,356 March 19, 1996
Grosveld et al. (Grosveld) 5,635,355 June 3, 1997
Chung 6,644,421 Sept. 3, 2002
NEB Catalog, pp. 32, 46, 48, and 83 (1995)
Grounds of Rejection
Claims 123-128, 130, 135, 143-145, and 147-151 stand rejected under 35 U.S.C.
§ 103(a) over Grosveld.
Claims 129, 131-133, and 152 stand rejected under 35 U.S.C. § 103(a) over
Grosveld taken with Li.
Claims 136-142 stand rejected under 35 U.S.C. § 103(a) over Grosveld taken
with NEB catalog (1995).
Claims 134 and 146 stand rejected under 35 U.S.C. § 103(a) over Grosveld
taken with Chung.
We reverse these rejections.
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