Appeal No. 2006-3250 Page 2
Application No. 10/468,562
b) passing the D-pantothenate-containing fermentation solution through
an anion exchanger and thereby binding the D-pantothenic acid to the
anion exchanger,
c) removing the D-pantothenic acid from the anion exchanger by eluting
the D-pantothenate bound to the anion exchanger in the form of calcium
and/or magnesium D-pantothenate by a solution containing inorganic or
organic calcium salts and/or magnesium salts, or eluting the bound
D-pantothenate in the form of free D-pantothenic acid using an HCl
solution,
d) optionally adding calcium base and/or magnesium base to set the
free D-pantothenic acid-containing eluate to a pH of 3-10, a solution
being obtained which contains calcium and/or magnesium pantothenate
and
e) subjecting the eluate or the solution containing calcium pantothenate
and/or magnesium pantothenate to drying and/or formulation
wherein no free β-alanine and/or β-alanine salt is fed to the culture
medium.
The references relied upon by the Examiner are:
Hikichi et al. (Hikichi) 5,518,906 May 21, 1996
Binder et al. (Binder) 6,582,939 Jun. 24, 2003
Hoffman-La Roche & Co. GB 562,267 Jun. 26, 1944
Baigori et al. (Baigori), “Isolation and Characterization of Bacillus subtilis Mutants
Blocked in the Synthesis of Pantothenic Acid,” J Bacteriology, Vol. 173,
No. 13, pp. 4240-4242 (1991)
Sorokin et al. (Sorokin), “Sequence analysis of the Bacillus subtilis chromosome
region between the serA and kdg loci cloned in a yeast artificial chromosome,”
Microbiol., Vol. 142, pp. 2005-2016 (1996)
GROUND OF REJECTION
Claims 1 and 5-19 stand rejected under 35 U.S.C. § 103 as being
unpatentable over the combination of Hikichi, Baigori, Binder and Hoffman-La
Roche.
Page: Previous 1 2 3 4 5 6 7 8 9 10 11 12 Next
Last modified: September 9, 2013