Appeal 2007-2400
Application 10/418,182
subset library for each of the possible combinations of three of the six
complementarity-determining regions, thereby totaling 20 subset
libraries;
e) subset libraries comprising mutated immunoglobulins in which the
predetermined amino acid has been substituted in at least one position
in four of the six complementarity-determining regions, with one
subset library for each of the possible combinations of four of the six
complementarity-determining regions, thereby totaling 15 subset
libraries;
f) subset libraries comprising mutated immunoglobulins in which the
predetermined amino acid has been substituted in at least one position
in five of the six complementarity-determining regions, with one
subset library for each of the possible combinations of five of the six
complementarity-determining regions, thereby totaling 6 subset
libraries; and
g) one subset library comprising mutated immunoglobulins in which the
predetermined amino acid has been substituted in at least one position
in all of the six complementarity-determining regions,
wherein each subset library that contains mutated immunoglobulins,
comprises mutated immunoglobulins in which the predetermined amino acid
is present at least once at every position in the complementarity-determining
region into which the predetermined amino acid has been introduced.
Claim 1 is directed to one of the “single predetermined amino acid
libraries” described on pages 15-16 of the Specification. (The Examiner
indicated in the Answer that a previous restriction requirement was
withdrawn, so “the library comprises all the subsets rather than a single
subset.” Answer 3.)
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