Appeal No. 94-3255
Application 07/673,264
present
invention, auxotrophic bacteria of a biologically pure
strain of type I methylotrophic Bacillus excrete
substantial
amounts of lysine. In a preferred embodiment we have
observed an amino acid auxotroph excreting from about
3 - 10 grams/per liter L-lysine [(Spec., p. 4, lines
12-17)].
. . .
Primary characteristics of the bacterium of the
present
invention are that it grows at a temperature of at least
50 C in an aqueous nutrient media that includes methanol0
as
a sole carbon and energy source with biotin, and vitamin
B12
s a required vitamins [sic][(Spec., p. 9, lines 23-27)].
. . .
Growth requires biotin in amounts from about 20 ug 1 to. -1
20 mg 1 . When grown in minimal salts media with. -1
methanol,
11
Page: Previous 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 Next
Last modified: November 3, 2007