Appeal No. 94-3255
Application 07/673,264
vitamin B and biotin the bacterium of the present
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invention can grow at a rate from about 0.2 hr to about-1
-1 E E
1.5 hr . at a temperature of about 50 C to 60 C [(Spec.,
p. 10, lines 27-31)].
. . .
It is envisioned that the present invention can be
employed
to produce amino acid auxotroph and/or amino acid analog
resistant mutants of the type I methylotrophic bacterium
of the genus Bacillus described herein that are capable of
producing most, if not all, of the known amino acids.
[(Spec. p. 13, lines 5-10)].
To produce amino acids from auxotrophic and/or amino
acid resistant mutants of the type I methylotrophic
Bacillus
of the present invention, the organism is cultured in an
aqueous nutrient medium having biotin, vitamin B , and
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methanol together with amounts of a phosphate source,
a sulfate source, a nitrogen source, calcium and trace
elements in amounts such as indicated in Example 4.
[(Spec. p. 13, lines 11-17)].
. . .
At a minimum, at least about 0.05% wt/vol. methanol,
. -1 . -1 . -1
0.5 ug 1 vitamin B and about 20 ug 1 to about 20 mg 1
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biotin are needed for mutant production of amino acids
[(Spec., p. 13, lines 25-28)].
. . .
Employing auxotrophs and/or amino acid resistant
mutants of the type I methylotrophic bacterium of the
present invention it is believed that amino acids can be
produced in substantial quantities. That is, quantities
of amino acids from at least 5 grams 1 . . . can be. -1
produced. While the present invention is believed useful
to produce many of the 20 amino acids, it is especially
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