Appeal No. 94-3255
Application 07/673,264
useful to produce lysine, phenylalanine, and tryptophan
either singly or simultaneously. In one embodiment,
auxotrophs which are also amino acid sensitive can
produce
from about 3 to about 5 grams 1 of lysine. In a. -1
preferred
embodiment, auxotrophs which are also amino acid
sensitive
can produce up to 8 grams/l L-lysine. Simultaneous
production of at least 4.0g 1 of L-lysine and at least. -1
1.5g 1 of L-aspartic acid can also be obtained. In one. -1
preferred embodiment, simultaneous production of 4.5g 1. -1
of L-lysine and 2.0g 1 of L-aspartic acid are obtained. -1
[(Spec., p. 14, line 15, to p. 15, line 1)].
The auxotrophic bacterium of Claim 55 must exhibit
“sustained growth at 50 C in nutrient media comprisingE
methanol as a source of carbon and energy and vitamin B ”
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(emphasis added) and excrete “at least about 5 g/l of lysine,
aspartic acid, phenylalanine, or tryptophan when grown on a
media containing a nitrogen source” (emphasis added).
However:
(1) The specification teaches that appellants’ novel
amino acid producing auxotrophic bacterium requires biotin to
grow (Spec., p. 10, line 27). Claim 55 appears also to be
directed to amino acid producing auxotrophic bacteria which
exhibit sustained growth in a nutrient media which does not
contain biotin.
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