Ex parte HANSON et al. - Page 43




          Appeal No. 94-3255                                                          
          Application 07/673,264                                                      


               (2) The specification teaches that appellants’ novel                   
          amino acid producing auxotrophic bacterium grows at a                       
          temperature of at least 50 C “in an aqueous nutrient media0                                                 
          that includes methanol as a sole carbon and energy source with              
          biotin, and vitamin B " (Spec., p. 9, lines 24-26).  Claim 55               
                               12                                                     
          appears also to be directed to amino acid producing                         
          auxotrophic bacteria which exhibit sustained growth in a                    
          nutrient media “comprising methanol,” i.e., in a nutrient                   
          media that includes “carbon and energy sources for growth                   
          other than methanol; including glucose or mannitol” (Spec., p.              
          10, lines 5-6).                                                             
               (3) The specification appears to teach that appellants’                
          novel amino acid producing auxotrophic bacteria are especially              
          useful to produce lysine, phenylalanine, and tryptophan singly              
          or simultaneously in quantities from at least 5 grams 1. -1                   
          (Spec.,                                                                     
          p. 14, lines 15-25) in nutrient media comprising methanol as a              
          sole carbon and energy source with biotin, and vitamin B .                  
                                                                  12                  
          Claim 55 appears to encompass amino acid producing auxotrophic              
          bacteria which produce at least about 5 g/l of lysine or any                
          amount of aspartic acid, phenylalanine or tryptophan, or at                 
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