Appeal No. 94-3255 Application 07/673,264 (2) The specification teaches that appellants’ novel amino acid producing auxotrophic bacterium grows at a temperature of at least 50 C “in an aqueous nutrient media0 that includes methanol as a sole carbon and energy source with biotin, and vitamin B " (Spec., p. 9, lines 24-26). Claim 55 12 appears also to be directed to amino acid producing auxotrophic bacteria which exhibit sustained growth in a nutrient media “comprising methanol,” i.e., in a nutrient media that includes “carbon and energy sources for growth other than methanol; including glucose or mannitol” (Spec., p. 10, lines 5-6). (3) The specification appears to teach that appellants’ novel amino acid producing auxotrophic bacteria are especially useful to produce lysine, phenylalanine, and tryptophan singly or simultaneously in quantities from at least 5 grams 1. -1 (Spec., p. 14, lines 15-25) in nutrient media comprising methanol as a sole carbon and energy source with biotin, and vitamin B . 12 Claim 55 appears to encompass amino acid producing auxotrophic bacteria which produce at least about 5 g/l of lysine or any amount of aspartic acid, phenylalanine or tryptophan, or at 14Page: Previous 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 NextLast modified: November 3, 2007