Appeal No. 95-4404
Application 07/913,121
(F) detecting and/or quantitating a label in a labeled cDNA complex, wherein
said label is introduced into said complex from labeled oligonucleotide primer, labeled
oligonucleotide probe or a labeled third oligonucleotide when the primer is unmodified,
and further wherein the amount of label is a measure of the resistance of the reverse
transcriptase to the drug or drugs evaluated at the concentration tested.
The references relied upon by the examiner are:
Mizoguichi 0,392,459 Oct. 17, 1990
(European Patent Document)
Holmes WO 90/06044 Jun. 14, 1990
Urdea et al, (Urdea), "A comparison of non-radioisotopic hybridization assay methods
using fluorescent, chemiluminescent and enzyme labeled synthetic
oligodeoxyribonucleotide probes", Nucleic Acids Research, vol. 16, no. 11, pp. 4937-4956
(1988).
Goff, "Retroviral Reverse Transcriptase: Synthesis, Structure, and Function", Journal of
Acquired Immune Deficiency Syndromes, vol. 3, pp. 817-831 (1990).
Laquel et al., (Laquel), "Wheat embryo DNA polymerase A reverse transcribes natural and
synthetic RNA templates. Biochemical characterization and comparison with animal DNA
polymerase p and retroviral reverse transcriptase", Biochem. Biophys. Acta, vol. 1048, pp.
139-148 (1990).
Mogensen et al. (Mogensen), "Nonradioactive, Sequence-Specific Detection of RNA in
Situ by Primed in Situ Labeling (PRINS)", Experimental Cell Research, vol. 196, pp. 92-98
(1991).
Konig et al., (Konig), "A Highly Sensitive Non-Radioactive Microassay For HIV-1 Reverse
Transcriptase", Journal of Cell Cellular Biochemistry, suppl. 16E, pg. Q222 (March 27,
-4-
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