Appeal No. 95-4404
Application 07/913,121
degrading the RNA from the duplex to form free cDNA; hybridizing the cDNA with a
chemically modified probe or probes to form cDNA-probe(s) complex capable of being
captured and/or detected; separating the cDNA-probe(s) complex from unreacted
probe(s); and detecting the presence of reverse transcriptase by detecting the cDNA-
probe(s) complex. Claims 8-13 are directed to an alternative embodiment wherein drug
resistant forms of reverse transcriptases are detected by performing the method in the
presence of a known reverse transcriptase inhibitor. Claims 14-19 are directed to yet
another embodiment wherein reverse transcriptase inhibitors are identified by performing
the method in the presence of candidate compounds.
Mizoguchi
The examiner has rejected all of the claims as obvious over Mizoguchi in
combination with Mogensen, Holmes, Urdea and Goff, and in the alternative, as obvious
over Mizoguchi in combination with Laquel, Holmes, Urdea and Goff.
Mizoguchi discloses a method of detecting reverse transcriptase in a biological
sample wherein a synthetic adenine ribopolynucleotide RNA (poly A) template is incubated
with an oligodeoxythyminenucleotide (oligo dT) primer to form a template-primer complex;
the template-primer complex is incubated with the biological sample and labeled
nucleotide substrate, allowing synthesis of a labeled cDNA-RNA duplex if reverse
transcriptase is present in the sample; unreacted template and substrate are separated
-7-
Page: Previous 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Next
Last modified: November 3, 2007