Appeal No. 1996-1042
Application No. 07/810,138
Claims 9 and 14, which are illustrative of the subject
matter on appeal, read as follows:
9. A biologically pure culture of asporogenous strain
Bacillus subtilis SMS275, suitable for use as a host component
in a host-vector system, characterized in that said stain
[sic] has a frequency of reversion to spore formers of less
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than 10 , plasmid stability, genetic markers leu, pyrD1, apr ,
npr and spoII:D [sic], and is deposited as CBS 432.90.-
14. A process for the production of a heterologous
polypeptide or protein or a precursor thereof, comprising the
steps of:
a) transforming an asporogenous strain Bacillus subtilis
SMS275 with a plasmid vector containing a gene coding for a
heterologous polypeptide or protein or a precursor thereof,
wherein said asporogenous strain Bacillus subtilis SMS275
is characterized in that it has a frequency of reversion to
spore formers of less than 10 , plasmid stability, genetic-8
- -
markers leu, pyrD1, apr , npr and spoII:D [sic], and is
deposited as CBS 432.90;
b) culturing the transformed asporogenous strain Bacillus
subtilis of step (a) in a suitable culture medium containing a
carbon source, a nitrogen source, mineral salts, leucine and
uracil; and
(c) recovering said heterologous polypeptide or protein
or a precursor thereof.
The references relied on by the examiner are:
Young et al. (Young) 4,302,544 Nov. 24, 1981
Dean et al. (Dean '235) 4,450,235 May 22, 1984
Dean et al. (Dean '236) 4,450,236 May 22, 1984
Dean et al. (Dean '773) 4,465,773 Aug. 14, 1984
Furutani et al. (Furutani) 5,015,574 May 14, 1991
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