Appeal No. 1996-1960
Application No. 07/975,167
wherein X means a halogen atom, m means an integer of 0 to 4, Y means hydroxy group, an alkoxy
group, a carboxyl group or sulfonic acid group, n means 0 or 1 and Z means nitro group or nitrovinyl
group.
16. A reagent for measuring lipase activity comprising a substrate as claimed in claim 15 and an
auxiliary enzyme.
17. A reagent for measuring lipase activity as claimed in claim 16 wherein the sugar ester of fatty
acid is a compound in which the aglycon is bonded in the %-type, and the auxiliary enzyme is %-
glucosidase alone or a combination of %-glucosidase and glucoamylase.
18. A reagent for measuring lipase activity as claimed in claim 16 wherein the sugar ester of fatty
acid is a compound in which the aglycon is bonded in the $-type and the auxiliary enzyme is $-
glucosidase alone, a combination of %-glucosidase and $-glucosidase or a combination of %-
glucosidase, glucoamylase and $-glucosidase.
19. A reagent for measuring lipase activity as claimed in claim 15 in which the sugar ester of
fatty acid represented by the formula (I) is selected from among the following compounds:
(1) 2-Fluoro-4-nitrophenyl 6-0-oleoyl-$-D-glucopyranoside
(2) 4-Nitrophenyl 6-0-palmitoyl-% -D-glucopyranoside
(3) 4-Nitrophenyl 4-0-acetyl-6-0linoloyl-0-% -D-gluco-pyranosyl-(164)-%-D-
glucopyranoside
(4) 2,3-difluoro-4-nitrophenyl 6-0-lauroyl-0-%-D-gluco-pyranosyl-(164)-$-D-
glucopyranoside
(5) 4-Nitrophenyl 6-0-pentanoyl-0-%-D-glucopyranoside
(6) 4-Nitrophenyl 4,6-D-dipentanoyl-0-%-D-glucopyranoside
20. A method for measuring lipase activity which comprises allowing a sample to act on a sugar
ester of fatty acid represented by the formula (I)
GĻ O-R (I)
3
wherein G stands for a group of the formula (A)
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