Appeal No. 1996-1960
Application No. 07/975,167
April 13, 1995), to the examiner’s supplemental answer (Paper No. 40, mailed August 29, 1995)
and to the examiner’s second supplemental answer (Paper No. 42, mailed January 23, 1996) for the
examiner’s reasoning in support of the rejection and to the appellants’ brief (Paper No. 36, filed
January 4, 1995), to the appellants’ reply brief (Paper No. 39, filed June 13, 1995) and to the
appellants’ second reply brief (Paper No. 41, filed October 30, 1995) for the appellants’ arguments
thereagainst.4
The claimed invention is directed to
C water-soluble G ~ OR sugar ester derivatives per se which are 4-nitrophenyl or 4-
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(nitrovinyl)phenyl-glucosides (i.e., formula (I)(B)(C)) or 4-nitrophenyl or 4-(nitrovinyl)phenyl-
maltosides (i.e., formula (I)(A)(C)) in which the hydroxyl group(s) at the 4- and/or 6-position
of the non-reductive terminal glucose is modified with an ester residue of saturated or
unsaturated fatty acid having 5-30 carbon atoms (claims 13-14),
C reagents for measuring lipase activity comprising the G ~ OR sugar ester derivative (claims 15
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and 19), further comprising at least one auxiliary enzyme selected from "-glucosidase,
glucoamylase and ß-glucosidase (claims 16-18), and
C a method for measuring lipase activity comprising allowing a sample to act on the
G ~ OR sugar ester derivative in the presence of at least one auxiliary enzyme selected from
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"-glucosidase, glucoamylase and ß-glucosidase to produce cleaved 4-nitrophenol or 4-
(nitrovinyl)phenol compounds, which compounds are then measured to determine the lipase
activity of the sample (claim 20).
(See specification, pages 4-5 and 13-14; brief, pages 2-4.)
4 Appellants’ third reply brief (Paper No. 43, filed March 25, 1996) was refused entry by the examiner in a
letter mailed May 8, 1996 (Paper No. 44) “because it is not limited to new points of argument or to new grounds of
rejection raised in the examiner’s answer” and, therefore, has not been considered in our decision.
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