Appeal No. 2001-1412 Paper No. 29
Application No. 08/629,177 Page 11
APPENDIX
1. A method for performing a non-competitive heterogeneous
immunoassay comprising an analyte specific component, an insulating layer,
and a fluorescently labelled analyte specific component comprising the steps
of
(a) immobilizing said analyte specific component 1) directly on a reaction
well or 2) on a solid phase attached to a reaction well wherein said analyte
specific component is in a single continuous region of said reaction well,
(b) adding an insulating layer which separates the analyte specific
component from the labelled analyte specific component, which labelled
analyte specific component can be antibodies or antigens, before drying,
(c) adding said labelled analyte specific component,
(d) drying said analyte specific component, said insulating layer, and said
labelled analyte specific component in said reaction well,
followed by the steps of:
(e) adding a sample containing an unknown amount of the analyte to be
analyzed,
(f) allowing said analyte to react with said analyte specific component and
said labelled analyte specific component,
(g) washing said well following reaction of said analyte with said analyte
specific component and said labelled analyte specific component, and
(h) detecting a signal from said labelled analyte specific component;
wherein all reagents other than said sample containing said analyte are
added prior to step (e) and wherein said signal is a measure of said
unknown amount of analyte in said sample.
2. A method for performing a non-competitive heterogeneous
immunoassay comprising an analyte specific component, an insulating layer,
and a fluorescently labelled analyte specific component comprising the steps
of
(a) adding a sample containing an unknown amount of analyte to be
Page: Previous 1 2 3 4 5 6 7 8 9 10 11 12 13 Next
Last modified: November 3, 2007