Appeal No. 2001-1412 Paper No. 29
Application No. 08/629,177 Page 12
analyzed to a device comprising: (1) a reaction well; (2) an analyte
specific component immobilized directly on a reaction well or on a solid phase
attached to said reaction well wherein said analyte specific component is in a
single continuous region of said reaction well; (3) a labelled analyte specific
component; and (4) an insulating layer which separates the analyte specific
component from the labelled analyte specific component, wherein said analyte
specific component immobilized on a solid phase, said labelled analyte specific
component and said insulating layer have been dried in said well,
(b) allowing said analyte to react with said analyte specific component and
said labelled analyte specific component,
(c) washing said well following reaction of said analyte with said analyte
specific component and said labelled analyte specific component, and
(d) detecting a signal from said labelled analyte specific component,
wherein all reagents necessary to perform said noncompetitive
immunoassay are immobilized or dried on said device prior to adding said
sample and wherein said signal is a measure of said unknown amount of
analyte in said sample.
15. A device for use in a non-competitive immunoassay comprising
(a) a reaction well,
(b) an analyte specific component immobilized on the surface of the
reaction well, either directly or via secondary immobilizing reagents, wherein
said analyte specific component is immobilized such that it will not wash off
from said reaction well and wherein said analyte specific component is in a
single continuous region of said reaction well,
(c) a fluorescently labelled analyte specific component, and
(d) an insulating layer that separates the analyte specific component from
the labelled analyte specific component, in which device the analyte specific
component, the labelled analyte specific component and the insulating layer
all have been dried.
Independent claims 28, 29 and 40 recite corresponding method and device claims
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