Interference 102,728
4. Brake, inter alia, pointed to the disclosure at p. 3, line 30- p. 4, line 27,
and original claim 5. Paper No. 15, pp. 6-7 and 9.
5. The ‘325 Application (pp. 3-4) states:
The constructs of the subject invention will have at least the following
formula defining a pro-polypeptide:
((R)r -(GAXYCX)n -Gene*)y
wherein:
R is CGX or AZZ, the codons coding for lysine and arginine, each of the
Rs being the same or different;
r is an integer of from 2 to 4, usually 2 to 3, preferably 2;
X is any of the four nucleotides, T, G, C, or A;
Y is G or C;
y is an integer of at least one and usually not more than 10, or usually not
more than four, providing for monomers and multimers;
Z is A or G; and
Gene* is a gene other than "-factor, usually foreign to a yeast host,
usually a heterologous gene, desirably a plant or mammalian gene;
n is 0 or an integer which will generally vary from 1 to 4, usually 2 to 3.
The pro-polypeptide has an N-terminal processing signal for peptidase
removal of the amino acids preceding the amino acids coded for by the Gene*.
For the most part, the constructs of the subject invention will have at
least the following formula:
L-(R-S-(GAXYCX)n )-Gene*)y
defining a pre-pro-polypeptide, wherein all the symbols except L and
S have been defined, S having the same definition as R, there being 1R and
1S, and L is a leader sequence providing for secretion of the pre-pro-
polypeptide. ... [emphasis added].
8
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