Appeal No. 1995-2789
Application No. 07/788,114
Claims 1 and 7 are representative of the subject matter on appeal and are
reproduced below:
1. A method for the production of threonine comprising
constructing a gene isolated from a genome selected from the
group consisting of Corynebacterium glutamicum, Escherichia coli, and
Bacillus subtilis, encoding an enzymatically active homoserine
dehydrogenase not subject to allosteric inhibition by threonine, wherein
the homoserine dehydrogenase gene is mutated at the carboxy
terminus, and
expressing the gene.
7. An enzymatically active homoserine dehydrogenase, isolated from
bacteria selected from the group consisting of Corynebacterium
glutamicum, Escherichia coli, and Bacillus subtilis, not subject to
allosteric inhibition by threonine, wherein the enzyme is altered at the
carboxy terminus.
The references relied upon by the examiner are:
Ernst-L. Winnacker (Winnacker), Directed Mutagenesis, in FROM GENES to
CLONES, INTRODUCTION to GENE TECHNOLOGY, 452-481 (Horst Ibelgaufts trans.,
VCH 1987)
Follettie et al. (Follettie), “Metabolic Engineering of Corynebacteria,” Proceedings
of the sixth International Symposium on Genetics of Industrial Microorganisms,
pp. 315-325 (1990)
Reinscheid et al. (Reinscheid), “Analysis of a Corynebacterium glutamicum hom
Gene Coding for a Feedback-Resistant Homoserine Dehydrogenase,”
J. Bacteriology, Vol. 173, pp. 3228-3230 (1991)
2
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