Appeal No. 1997-2510
Application No. 07/868,539
Claims 1 and 6 are illustrative of the subject matter on appeal and are
reproduced below:
1. A method of inhibiting replication of a virus in an infected cell in vivo,
comprising
selecting a DNA fragment having (i) a first region having 6-30
bases whose sequence corresponds to a sequence of DNA
recognized by a viral-specific transcription factor, (ii) a second region
having a sequence of nucleotides complementary to said first region,
when said first and second regions are positioned in an anti-parallel
configuration, and (iii) joining said first and said second regions in a 5’
to 3’ direction, a tetranucleotide sequence X1X2X3X4, where X1 is U or
T, and X2 is U, T, G, A or C, and X3 is C, and X4 is G (SEQ ID NO: 14);
or X1 is G, and X2 is U, T, G, A or C, and X3 is G or A, and X4 is A
(SEQ ID NO: 15); or X1 is C, and X2 is U or T, and X3 is U or T, and X4
is G (SEQ ID NO: 16), and
introducing the fragment into the cell in an amount sufficient to
inhibit replication of the virus in the cell.
6. A pharmaceutical composition for treating a virus infection, comprising
a pharmaceutical excipient containing a DNA fragment having (i) a first
region having a 5’ terminus and a 3’ terminus, said region having 6-30
bases whose sequence corresponds to a sequence of DNA
recognized by a viral-specific transcription factor, (ii) a second region
having a 5’ terminus and a 3’ terminus, said region having a sequence
of nucleotides complementary to said first region when said first and
second regions are positioned in an anti-parallel configuration, and (iii)
a covalent link between the 5’ terminus of one region and the 3’
terminus of the other region.
The references relied upon by the examiner are:
Kaji 4,689,320 Aug. 25, 1987
Summerton et al. (Summerton) 5,142,047 Aug. 25, 1992
Inouye 5,208,149 May 4, 1993
Metzler, “Biochemistry: The Chemical Reactions of Living Cells,” Academic
Press Inc., p. 103 (1977)
Snedecor et al. (Snedecor), “Statistical Methods,” Iowa State University
Press/Ames, 8th Edition, pp. 10-13, 26-37 (1989)
Uhlmann et al. (Uhlmann), ”Antisense Oligonucleotides : A New Therapeutic
Principle,” Chemical Reviews, Vol. 90, No. 4, pp. 544-579 (1990)
2
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