Interference 103,579
Sequenced molecule: genomic DNA
Name: GBSS gene from potato
Length of sequence: 4964 bp
Hofvander’s involved application, filed November 24, 1993, as
amended November 17, 1993, further characterizes SEQ ID No. 5 as
follows:
(2) INFORMATION FOR SEQ ID NO:5:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 4964 base pairs
(B) TYPE: nucleic acid
(C) STRANDEDNESS: single
(D) TOPOLOGY: linear
(ii) MOLECULE TYPE: DNA (genomic)
Hofvander’s PCT application (p. 3, l. 21-24) and Hofvander’s
involved application (p. 3, l. 21-24)(HR 277) state, “The gene
for potato GBSS has . . . so far not been characterised [sic] to
the same extent as the waxy gene in maize, either with respect of
locating or structure.” Regarding the state of the art with
respect to the potato GBSS gene and its use for inhibiting
amylose production in potato plant tubers, Hofvander’s involved
application teaches (p. 4, l. 28, to p. 5, l 22 (HR 278-279);
emphasis added):
In potato, experiments have previously been made
to inhibit the synthesis of the granule-bound starch
synthase (GBSS protein) with an antisense construct
corresponding to the gene coding for GBSS (this gene is
hereafter called the “GBSS gene”). Hergersberg (1988)
[(VDX 1)] describes a method by which a cDNA clone for the
GBSS gene in potato has been isolated by means of a cDNA
clone for the wx+ gene in maize. An antisense construct
based on the entire cDNA clone was transferred to leaf
discs of potato by means of Agrobacterium tumefaciens.
-31-
Page: Previous 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 Next
Last modified: November 3, 2007