Interference 103,579
In microtubers induced in vitro from regenerated potato
sprouts, a varying and very weak reduction of the amylose
content was observed and shown in a diagram. A complete
characterization of the GBSS gene is not provided.
The gene for the GBSS protein in potato has been
further characterised [sic] in that a genomic wx+ clone
was examined by restriction analysis. However, the DNA
sequence of the clone has not been determined (Visser
et al, 1989[7]).
Further experiments with an antisense construct
corresponding to the GBSS gene in potato have been
reported. The antisense construct which is based
on a cDNA clone together with CaMV 35S promoter has
been transformed by means of Agrobacterium rhizogenes.
According to information, the transformation resulted
in a lower amylose content in the potato, but no values
have been accounted for (Flavell, 1990).
None of the methods used so far for genetically
engineered modification of potato has resulted in potato
with practically no amylose-type starch.
The object of the invention therefore is to provide
a practically complete suppression of the formation of
amylose in potato tubers.
According to Hofvander’s involved application (p. 5, l. 30,
to p. 6, l. 20 (HR 279-280); emphasis added):
The antisense constructs according to the invention
comprise both coding and noncoding parts of the GBSS gene
which correspond to sequences in the region comprising
promoter as well as leader sequence, translation start,
translation end and trailer sequence in the antisense
direction. For a tissue-specific expression, i.e. the
7 Visser, R.G.F., Hergersberger, M., van der Leij, F.R.,
Jacobsen, E., Witholt, B. and Feenstra, W.J. (Visser’s 1989
publication), “Molecular Cloning and Partial Characterization of
the Gene for Granule-Bound Starch Synthase from a Wildtype and
an Amylose-Free Potato (Solanum Tuberosum L.),” Plant Science,
Vol. 64, pp. 185-192 (1989)(Appendix A).
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