Interference 103,579
amylose production should be inhibited in the potato tubers
only, use is made of promoters which are specifically
active in the potato tuber. As a result, the starch
composition in other parts of the plant is not affected,
which otherwise would give negative side-effects.
The invention thus comprises a fragment which
essentially has one of the nucleotide sequences stated in
SEQ ID No. 1, SEQ ID No. 2 or SEQ ID No. 3. However, the
sequences may deviate from those stated by one or more
non-adjacent base pairs, without affecting the function
of the fragments.
The invention also comprises a potato-tuber-specific
promoter comprising 987 bp which belongs to the gene
according to the invention, which codes for granule-bound
starch synthase. Neither the promoter nor the corresponding
gene has previously been characterised [sic]. The promoter
sequence is stated in SEQ ID No. 4, while the gene sequence
is stated in SEQ ID No. 5. Also the promoter and gene
sequences may deviate from those stated by one or more
non-adjacent base pairs, without affecting their function.
Hofvander’s involved application describes its Figure 2
(HR 312; VDX 10) as follows (HR 281, l. 1-2), “Fig. 2 shows
the result of restriction analysis of the potato GBSS gene.”
The specification of Hofvander’s involved application explains
how the Figure 2 GBSS gene was characterized (HR 282, l. 1-24):
A full-length clone of the potato GBSS gene, wx311,
has been identified and isolated from the genomic library.
The start of the GBSS gene has been determined at an EcoRI
fragment which is called fragment w (3.95 kb). The end of
the GBSS gene has also been determined at an EcoRI fragment
which is called fragment X (5.0 kb). A BgIII-SpeI fragment
which is called fragment m (3.9 kb) has also been isolated
and shares sequences both from fragment w and from fragment
x. The fragments w, m and x have been subcloned in pUC13
(Viera, 1982; Yanisch-Peron et al, 1985) and are called pSw,
pSm and pSx, respectively (Fig. 2).
The GBSS gene in potato has been characterized
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