Interference 103,579
The object of the invention therefore is to provide
a practically complete suppression of the formation of
amylose in potato tubers.
Hofvander’s involved application expressly states
(HR 279-280; emphasis added):
The antisense constructs according to the invention
comprise both coding and noncoding parts of the GBSS gene
which correspond to sequences in the region comprising
promoter as well as leader sequence, translation start,
translation end and trailer sequence in the antisense
direction. For a tissue-specific expression, i.e. the
amylose production should be inhibited in the potato tubers
only, use is made of promoters which are specifically
active in the potato tuber. As a result, the starch
composition in other parts of the plant is not affected,
which otherwise would give negative side-effects.
The invention thus comprises a fragment which
essentially has one of the nucleotide sequences stated in
SEQ ID No. 1, SEQ ID No. 2 or SEQ ID No. 3. However, the
sequences may deviate from those stated by one or more
non-adjacent base pairs, without affecting the function
of the fragments.
The invention also comprises a potato-tuber-specific
promoter comprising 987 bp which belongs to the gene
according to the invention, which codes for granule-bound
starch synthase. Neither the promoter nor the corresponding
gene has previously been characterised [sic]. The promoter
sequence is stated in SEQ ID No. 4, while the gene sequence
is stated in SEQ ID No. 5. Also the promoter and gene
sequences may deviate from those stated by one or more
non-adjacent base pairs, without affecting their function.
Similarly, as discussed previously, the claims,
specification, and prosecution history in Visser’s involved
application, and other extraneous evidence of record, establish
that “full length” PGBSS cDNA or genomic DNA coding for PGBSS in
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