Interference 103,579
Furthermore, it demonstrates that the GBSS fragment used
in pKGBA31, or at least part of it, is essential for the
inhibition of GBSS gene expression, as the inhibitory
effect of pGBA20, pKGBA20 and pKGBA25 was much lower.
For pGBA30 and pKGBA30, the weak inhibitory effect
may be caused by a premature transcription termination.
The genomic fragment used for these constructs contains a
3' non-GBSS sequence, which comprises a part of a putative
pseudogene (van der Leij et al. 1993), in addition to the
GBSS fragment that is also present in pKGBA31. . . . A
premature transcription stop does not necessarily result
in the absence of antisense inhibition, as has been
described for pGB50 (Kuipers et al. 1994) and several
other antisense genes . . . but in the case of pGBA30 and
pKGBA30 the resulting antisense RNA might lack sequences
that are complementary to the GBSS mRNA.
The variation in the inhibitory effects of the
partial genomic antisense constructs points towards a
function for certain regions of the gene in antisense
inhibition. . . . .
(c) Construct[s] “comprising” a fragment;
construct[s] “containing” a sequence;
and sequence[s] “comprise[s]” sequence[s]
The following phrases appear in the claims of Hofvander’s
involved application (emphasis added):
Hofvander’s Claim 1
“. . . a gene construct comprising a fragment of the potato
gene which codes for formation of granule-bound starch synthase
(GBSS gene) inserted in the antisense direction, wherein said
fragment is selected from the group consisting of SEQ ID No. 1,
SEQ ID No. 2 and SEQ ID No. 3, together with a promoter selected
from the group consisting of CAMV 35S, patatin I and the GBSS
promoter”;
-49-
Page: Previous 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 Next
Last modified: November 3, 2007