Interference 103,579
it also defines the antisense pGB50 vectors said in Visser’s
involved application to have been used to transform potato plants
(VR 154) and substantially inhibit the expression of PGBSS (VR
156-158) as constructs comprising GBSS cDNA which corresponds to
genomic SUB10. SUB10 is defined as a 3.0kb HindIII-SpeI fragment
of the GBSS genecontaining the complete coding region of the
GBSS gene in reverse orientation and an upstream 35S CaMV
promoter (Appendix B, p. 3).
The above additional information is particularly significant
because it lays a firm basis for interpreting the metes and
bounds of the phrase “full length potato . . . GBSS . . . cDNA or
genomic DNA sequence . . . in reverse orientation” in all claims
of Visser’s involved application. Figures 2A and 2B of Visser’s
Rule 132 declaration (Appendix B, last page) depict what
reasonably appears to be the same LGBSSwt-6 clone, the same gene
including the 5' promoter and the 3' terminator regions, and the
same GBSS gene fragments SUB10, SUB20, SUB25, SUB30, and SUB31
which form the same pGB50, pKGBA50, pGBA10, pKGBA10, pGBA20,
pKGBA20, pKGBA25, pGBA30, pKGBA30, pKGBA31 gene constructs
depicted in Figures 1A and 1B (VDX 4, p. 748) of Kuipers et al.
(Kuipers’ 1995 publication)8, “Factors Affecting the Inhibition
by Antisense RNA of Granule-Bound Starch Synthase Gene Expression
8 Named authors are Anja G.J. Kuipers, Wim J.J. Soppe,
Evert Jacobsen, and Richard G.F. Visser (VDX 4, p. 745).
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