Interference 103,579
of the promoter” (Appendix B, p. 2, first full para.). The
following is said to have been performed (Appendix B, pp. 2-4):
A series of eleven antisense constructs was made
based on GBSS cDNA and genomic sequences, the 35S CaMV
promoter and the GBSS promoter (Fig. 2). The construction
of pGB50 has been described before (Visser et al.,
Mol. Gen. Genet., 225:289-26 [sic 289-296] (1991))
[(VDX 8)]. . . . .
For the construction of pKGBA50 . . . the 2.2kb
BamHI-SpeI fragment from pGB2 . . . was ligated in
reversed orientation into digested pPGB-1S. For the
construction of pGBA10 and pKGBA10 the 3.0kb HindIII-
SpeI fragment containing the complete coding region
of the GBSS gene . . . was subcloned in pUC19 (=SUB10;
Fig. 2a). The BamHI-SpeI fragment of SUB10 was ligated
in reverse orientation into digested pBl121S or pPGB-1S,
respectively.
The partial genomic antisense constructs pGBA20,
pKGBA20, pGBA30 and pKGBA30 are based on BamHI and SstI
digested pBl121 and pPGB-1. The 1.8kb HindIII-Nsi1
fragment of the GBSS gene was subcloned in pMTL23 . . .
and isolated as an SstI-BamHI fragment (=SUB20; Fig. 2a).
This fragment was ligated in reversed orientation into
pBl121 (=pGBA20) and pPGB-1 (=pKGBA20). The 1.4kb SstI-
KpnI fragment of the GBSS gene was subcloned in pUC19
and isolated as an SstI-BamHI fragment (=SUB30; Fig. 2a),
which was ligated in reversed orientation into pBl121
(=pGBA30) and pPGB-1 (=pKGBA30). For construction of
pKGBA25 . . . PCR products were . . . restricted with
SstI and XbaI and ligated in reversed orientation into
XbaI-SstI digested pPGB-1. For the construction of
pKGBA31 the 0.6kb SstI-SpeI fragment of the GBSS gene
(=SUB31; Fig. 1a [sic, 2a]) was directly ligated in
reversed orientation into XbaI-SstI digested pPGB-1.
Accompanying Visser’s Declarations under 37 CFR § 1.132 is
Figure 2 (Appendix B, last page). Figure 2A is said to depict:
LGBSSwt-6: the “full length genomic clone” described
in Visser’s 1989 publication (Appendix A,
p. 188)(“The line on top indicates the gene
-44-
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