Interference 103,579
including the promoter region (5'dashed line)
and the terminator region (3' dashed line).)
(Appendix B);
SUB10: the 3.0kb HindIII-SpeI fragment of the GBSS gene
containing the complete coding region of the GBSS
gene subcloned in pUC19 (Appendix B, p. 3);
SUB20: the 1.8kb HindIII-Nsi1 fragment of the GBSS gene
subcloned in pMTL23 . . . and isolated as an SstI-
BamHI fragment (Appendix B, p. 3);
SUB25: a 1.1kb fragment of the GBSS gene amplified via
PCR with a 23-mer Sst-primer at the 5' end of the
fragment and a 23-mer Xba-primer at the 3' end of
the fragment (Appendix B, pp. 3-4);
SUB30: the 1.4kb SstI-KpnI fragment of the GBSS gene
subcloned in pUC19 and isolated as an SstI-BamHI
fragment (Appendix B, p. 3);
SUB31: the 0.6kb SstI-SpeI fragment of the GBSS gene.
Figure 2B depicts, inter alia, the following constructs
comprising GBSS cDNA and SUB10, SUB20, SUB25, SUB30, and SUB31
genomic DNA fragments in reverse orientation with a GBSS (GB) or
35 CaMV (35S) promoter:
35 CaMV (35S) promoter GBSS (GB) promoter
pGB50 35S-GBSS cDNA; pKGBA50 GB-GBSS cDNA;
pGBA10 35S-SUB10; pKGBA10 GB-SUB10;
pGBA20 35S-SUB20; pKGBA20 GB-SUB20;
pKGBA25 GB-SUB25;
pGBA30 35S-SUB30; pKGBA30 GB-SUB30;
pKGBA31 GB-SUB31.
While Visser’s Rule 132 declaration was expressly designed
to demonstrate unexpectedly superior expression of GBSS cDNA and
GBSS cDNA and genomic DNA fragments under the control of the
GBSS promoter as compared to control by the 35 CaMV promoter,
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