Appeal No. 2001-1378
Application No. 08/832,571
Claim 1 is representative of the claims on appeal and reads as follows.
1. An isolated polynucleotide sequence comprising:
(a) a first nucleotide sequence comprising a sequence selected from the group
consisting of:
(i) a nucleotide sequence encoding the p110 of PI 3-kinase protein and
(ii) a nucleotide sequence encoding a derivative or mutant of (a)(i) having single
or multiple nucleotide substitutions, deletions or additions, said sequence encoding said
derivative or mutant of (a)(i) having at least 50% identity to a native nucleotide
sequence encoding p110, said derivative or mutant having 60-90% sequence identity to
the native amino acid sequence of the p110 subunit of PI 3-kinase and an activity of the
p110 subunit of PI 3-kinase protein; and
(b) a second nucleotide sequence comprising a sequence encoding a cell
membrane targeting sequence, said second sequence being attached to the 5' or 3' end
of said first sequence.
The references relied upon by the examiner are:
Hu et al. (Hu), “Ras-Dependent Induction of Cellular Responses by Constitutively
Active Phosphatidylinositol-3 Kinase,” Science, Vol. 268, pp 100-102 (1995)
Kapeller et al. (Kapellar), “Phosphatidylinositol 3-Kinase, “ BioEssays, Vol. 16,
No. 8, pp. 565-576 (1994)
Varticovski et al. (Varticovski), “Activation of Phosphatidylinositol 3-Kinase in
Cells expressing abl Oncogene Variants,” Molecular and Cellular Biology, Vol. 11, No.
2, pp. 1107-1113 (1991)
Aronheim et al (Aronheim), “Membrane Targeting of the Nucleotide Exchange
Factor Sos is Sufficient for Activating the Ras Signaling Pathway,” Cell, Vol. 78, pp.
949-961 (1994)
Klippel et al. (Klippel 94), “The Interaction of Small Domains Between the
Subunits of Phosphatidylinositol 3-Kinase Determines Enzyme Activity,” Molecular and
Cellular Biology, Vol. 14, No. 4, pp. 2675-2685 (1994)
Klippel et al. (Klippel 93), “A Region of the 85-Kilodalton (kDa) Subunit of
Phosphatidyl-inositol 3-Kinase Binds the 110-kDa Catalytic Subunit In Vivo,” Molecular
and Cellular Biology, Vol. 13, No. 9, pp. 5560-5566 (1993)
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