Appeal No. 2001-1498 Page 2
Application No. 08/912,378
9. A composition for delivering a nucleic acid to a bacterial cell, the
composition comprising
the nucleic acid mixed with a macrocycle having a net positive
charge selected from the group consisting of natural porphyrins,
natural phthalocyanins, synthetic porphyrins, synthetic
phthalocyanins, and conjugates thereof, in an amount effective
to enhance delivery of the nucleic acid to the bacterial cell,
wherein the nucleic acid is a nucleic acid to be delivered to a
bacterial cell, wherein the nucleic acid is ionically bound to the
macrocycle.
We also note that claims 4 and 12 further limit the macrocycle of claims
1 and 9, respectively, to porphyrin. In addition, claim 13 further limits the
composition of claim 9 by requiring that the composition be made by mixing a
plurality of the porphyrin and a plurality of the nucleic acid in a ratio resulting in
all of the plurality of the nucleic acid binding to the plurality of the porphyrin.
The references relied upon by the examiner are:
Takle WO 95/27480 Oct. 19, 1995
Yuan WO 95/24489 Sept. 14,1995
Sessler WO 96/21665 Jul. 18, 1996
George WO 96/21731 Jul. 18, 1996
Ernst-L. Winnacker (Winnacker), From Genes to Clones Introduction to Gene
Technology 487-490 (Horst Ibelgaufts, trans., VCH 1987)
Gibbs et al. (Gibbs), “Interaction of Porphyrin and Metalloporphyrins with Nucleic
Acids,” Seminars in Hematology, Vol. 26, No. 2, pp. 77-85 (1989)
Ortigao, et al. (Ortigao), “Solid-phase Introduction and Intracellular Photoinduced
Reaction of a Water-soluble Meso-tetracarboxyporphine Conjugated to an
Antisense Oligodeoxyribonucleotide,” Biochimie, Vol. 75, pp. 29-34 (1993)
Kobayashi et al. (Kobayashi), “Reversal of Drug Sensitivity in Multidrug-Resistant
Tumor Cells by an MDR1 (PGY1) Ribozyme,” Cancer Res., Vol. 34, p. 1271-
1275 (1994)
Page: Previous 1 2 3 4 5 6 7 8 9 10 Next
Last modified: November 3, 2007