Interference No. 104,436 Paper 98
Shyamala v. IEllman Page 4
[201 Dr. Shyamala directed Mr. Khoja to screen thousands of proteins randomly selected from a
protein library to i dentify proteins interacting with MKK3 [1025 at 4-5; 10 1 02 at 31.
[211 The screening involved inserting test sequences of complementary deoxyribonucleic acid
(cDNA) into yeast to produce test proteins and identifying successful interactions of test proteins
w I ith MKK3 by a change in color [1025 at 4].
[22] According to Mr. Khoja, he analyzed the DNA of clones that demonstrated successful
interactions [1010 at 3].
[23] A 20 February 1996 entry in Mr. Khoja's Notebook No. 7613 shows photographs of two gels
sorting insert cDNAs by size [ 10 10 at 4; 10 12 at 137].
[24] According to Shyamala and Khoja, he identified several clones with 1.4 kilobase (kb) inserts of
cDNA as being of interest [ 10 10 at 4. 1025 at 6].
[251 According to Mr. Khoja, the run in the lower photograph labeled B73 proved to code for what
was later identified as MIP [fOIO at 41.
[26] Shyamala does not explain why, on 20 February 1996, 1.4 kb proteins were of particular interest
or what significance Shyamala or Khoja ascribed to B73 on that date.
[271 Mr. Khoja requested sequencing of clone 14b, which he states was B73 [1010 at 4).
(28] The notebook of Chun Ting Lee-Ng shows five forms labeled "Request for DNA Sequence"
from Hamid Khoja, one of which (top right) lists " 14B". The requests are initialed as received
26 February 1996 [1014' at 163].
2 Declaration of Harniduddin Kboja.
' Lee-Ng Notebook No. 7468.
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