Interference No. 104,436 Paper 98
Shyamala v. Hillman Page 5
[29] Mr. Khoja declared [1010 at 5] that he believes the computer-generated sequence [1015] titled
"Translation of DNA 14b-comp.seq" and dated 'Thu Mar 21 14:39:36 1996" is a print out of the
tested DNA for clone 14b with undated annotations in his handwriting and Dr. Shyarnala's
handwriting.
[30] Mr. Khoja states that "Dr. Shyamala and I determined that the insert in clone l4b encoded only
part of a protein, because the 14b insert did not contain sequences encoding the amino acid
methionine, which initiates all protein sequences" [1010 at 5].
[31] In the computer translation [ 10 15], all three of the computer-generated polypeptide sequences
have at least one methionine before a stop codon.
[32] According to Mr. Khoja, he isolated the remaining 5'cDNA sequence associated with the l4b
DNA sequence and submitted it to Ms. Lee-Ng for sequencing on 16 May 1996 [ 10 10 at 5-6;
10164at 49].
[33] Mr. Khoja states [ 1010 at 6] that the sequence listing titled " l4bcom ed Translated Sequence"
and dated 23 May 1996 [1017] is the sequence for one of the clones he submitted because it is
dated one week after he submitted the clones for sequencing, because it contains the I I missing
amino. acids, and because it has a 38 nucleotide overlap with the earlier partial sequence [10151.
[34] Mr. Khoja does not explain how he knows there were I I missing amino acids or why he believed
Exhibit 1017 shows the missing amino acids.
[35] Nucleotides 16-77 of the March sequence [ 10 15] overlap with nucleotides 200-261 of the May
sequence [1017].
4 Lee-Ng Notebook No. 8093.
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