Interference No. 104,436 Paper 98
Shyarnala v. Hillman Page 6
[36] According to Dr. Shyamala, the "com" notation in the title of the sequence indicates that it is
complete, and the "ed" notation means that the sequence was edited [ 1025 at 7].
[37] According to Mr. Khoja, the printout entitled "MIK nt Translated Sequence" dated 14 June 1996
[10181 was generated by aligning and then combining of the two earlier sequences.
[38] According to Mr. Kboja he prepared a fuU-length DNA clone at Dr. ShyamaIa's request, which
was then submitted for sequencing [ 10 10 at 7; 10 16 at 103).
[39] Mr. Khoja had also experimentally verified that the l4b clone interacted with MIM [1010 at 7
8; 10 12 at 1791.
[40] Mr. Khoja had also localized the DNA in question to human chromosome 14 [1010 at 8; 1012
at 175].
[41] Mr. Khoja determined, using western blot analysis, that the 14b protein had a molecular weight
of about 40 kilodaltons (kD) and noted a high level of expression for the protein. According to
Mr. Khoja, in this instance he was referring to the full-length l4b clone [1010 at 8; 1013 at 5].
[42] Mr. Khoja noted two undated BLAST alignments showing 76.7% and 79.2% homology between
the putative ME? sequence and two other known proteins: guanosine monophosphate reductase
(gmg) and glucose-6-phosphate dehydrogenase (hg6pd 1), respectively [ 10 10 at 9; 1020; 10 191.
[43] According to Dr. Shyamala, in May 1996 she further characterized the expression pattern for the,
partial l4b clone by using l4b cDNA hybridization with messenger ribonucleic acid (mRNA) to
identify MIP expression in various tissues [ 1025 at 10-11; 1024 at 43].
[44] Page 42 of Dr. Shyamala's notebook indicates that both l4b and "G3PDH" were to be analyzed
1024 at 42].
Page: Previous 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Next
Last modified: November 3, 2007