cells must not be serum starved. Either a cell from the culture or the nucleus of such a cell
is inserted into an enucleated oocyte of the same species as the cultured cell. Strelchenko
refers to the product so formed as a cybrid. The cybrid is cultured to form an embryo. The
embryo is then implanted in the uterus of an ungulate animal of the same species as the
original cultured cell to produce a fetus which develops into the ungulate animal.
F 10. Strelchenko's involved Claims 57 and 106 are representative of the interfering subject matter
as claimed by Strelchenko:
57. A method for preparing an ungulate animal, said method
comprising:
a) obtaining a cell from an ungulate fetus;
b) culturing said cell to form a cell culture;
C) forming a cybrid by nuclear transfer of a cell obtained from
said cell culture, or a nucleus thereof into an enucleated
oocyte obtained from the same species as the cell in step (a);
and
d) culturing said cybrid so as to generate an embryo comprising
embryonic cells; and
e) transferring said embryo of step (d) or a recloned embryo of
said embryo of step (d) into the uterus of an ungulate of the
same species as the cell in step (a) so as to produce a fetus
that undergoes full fetal development and parturition to
generate said ungulate animal.
106. A method for preparing an ungulate animal, said method comprising:
a) obtaining a non-embryonic cell from an ungulate;
b) culturing said non-embryonic cell to form a cell culture,
wherein said cell culture is not serum starved-,
C) forming a cybrid by nuclear transfer of a cell obtained from
said cell culture, or a nucleus thereof into an enucleated
oocyte obtained from the same species as the cell in step (a);
d) culturing said cybrid so as to generate an embryo comprising
embryonic cells; and
e) transferring said embryo of step (d) or a recloned embryo of
said embryo of step (d) into the uterus of an ungulate of the
same species as the cell in step (a) so as to produce a fetus
that undergoes full fetal development and parturition to
generate said ungulate animal.
Paper 10, pp. 2 and 4-5.
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