Interference 103,781
Dr. Adang found that Bt DNA contained the ATTTA sequences Shaw
found problematic to transcription (AR 0372-0374). Adang
especially relies (AB 50, Fact 125) on a memorandum he drafted to
Dr. Murray on September 20, 1986 (AR 0372-0374; AX 34C). The
handwritten memorandum, dated “9/20/86" and initialed “MJA”
reads, in part (AX 34C):
Liz,
Here are the searches of 4 bt genes for ATTTA in the
coding sense direction. It seems that one needs to
search plant 3' untranslated regions for AU sequences
to get a feeling for their role in plant mRNA
stability.
As you can see all 4 genes have AUUUA sequences in the
central 1/3 of the gene.
It is interesting that cycloheximide increased RNA to
normal levels. Can we do this w/ Bt constructs in
protoplasts? . . . .
We fail to see how Shaw’s disclosure remedies the defects in
Adang’s case. The evidence of record shows that Adang did not
understand and could not explain why Bt gene sequences in plants
were not being efficiently expressed in plants. The record shows
that there are significant differences between genetic sequences,
codon usage, etc. in Bacillus thuringiensis and plants, not to
mention the differences among various plants themselves. These
differences may be the cause of the problems Adang aimed to
solve. Regardless of how impressed or excited Drs. Adang and
Murray were by the teaching of Shaw’s publication prior to
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