Interference No. 105,188
Short v. Punnonnen
the encoded protein on an immune response as compared to the
response prior to alteration. The procedures described in the
Freeman PCT for obtaining new polynucleotides comprise the steps
of: a) creating a library comprising recombinant polynucleotides,
5 and b) screening the library to identify polynucleotides which
encode B7-1 and/or B7-2 variants having altered modulatory
effects on an immune response, whether the effects are enhanced
immune responses or inhibited immune responses to a vaccine
vector. For a description of construction of a cDNA library,
10 see the Freeman PCT at page 18, l. 27, to page 19, l. 20 (II.
Isolation of mRNA and Construction of cDNA Library); page 70,
l. 11, to page 72, l. 4 (Example 4: Cloning, Sequencing and
Expression of the B7-2 Antigen, A. Construction of cDNA Library);
and page 78, l. 17, page 80, l. 6 (Example 6, Cloning and
15 Sequencing of the Murine B7-2 Antigen, A. Construction of cDNA
Library). On consideration of more detailed instructions in the
Freeman PCT, we also find that the inventive procedures described
therein include recursive screenings for DNA encoding altered,
active B7-2 antigens (Exh. 2040, III. Transfection of Host Cells
20 and Screening for Novel B Lymphocyte Activation Antigens, p. 19,
l. 23, to p. 20, l. 6; emphasis added):
The thus prepared cDNA library is then used to clone
the gene of interest by expression cloning techniques.
. . . .
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