Interference No. 105,188
Short v. Punnonnen
We find that the hereinabove transcribed paragraphs of the
Short PCT describe methods for obtaining polynucleotides having
an optimized phenotype comprising: a) creating a library of
recombinant polynucleotides; and b) screening the library to
5 identify those polynucleotides having a variant phenotype,
whereby optimization is achieved by recursive sequence
recombination. The Short PCT adds (Exh. 2050, p. 40, ll. 5-18):
In some cases, it is desirable to bias the repertoire
for a preselected activity, such as by using as a source of
10 nucleic acid cells (source cells) from vertebrates in any
one of various stages of age, health and immune response.
For example, repeated immunization of a healthy animal prior
to collecting rearranged B cells results in obtaining a
repertoire enriched for gentic material producing a ligand
15 binding polypeptide of high affinity.
Accordingly, the foundation for our previous finding that
“the Short PCT fails to remedy the deficiencies of the Freeman
PCT [because] . . . Short has not shown that the Short PCT
20 describes using recursive sequence recombination to create
recombinant polynucleotide libraries as required by Punnonnon
claim 47" (Paper No. 181, p. 142, third full paragraph) did not
take into account the full scope and content of the teachings of
the Freeman PCT.
25 4. Stemmer
We find that, as of the date Punnonen’s involved application
was first filed, recursive sequence recombination was a procedure
-18-
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