Appeal 2007-1161
Application 09/954,166
were not provided. See 37 C.F.R. 41.37(c)(1)(vii). We select claim 43 as
representative for the purpose of deciding all the issues in this appeal. Claim
43 reads as follows:
43. A method of making a first molecular complex
comprising four fusion proteins, comprising the steps of:
expressing in a host cell a first fusion protein comprising
(a) an immunoglobulin heavy chain comprising a variable
region and (b) an extracellular domain of a first transmembrane
polypeptide; and
expressing in a host cell a second fusion protein
comprising (a) an immunoglobulin light chain comprising a
variable region and (b) an extracellular domain of a second
transmembrane polypeptide,
whereby two first and two second fusion proteins
associate to form a first molecular complex comprising at least
four fusion proteins, wherein the first molecular complex
comprises two ligand binding sites, each ligand binding site
formed by the extracellular domains of the first and second
transmembrane polypeptides, wherein the first molecular
complex has an affinity for a cognate ligand which is increased
relative to a second molecular complex consisting of the
extracellular domain of the first transmembrane polypeptide
and the extracellular domain of the second transmembrane
polypeptide.
FINDINGS OF FACT
1. T-cell antigen receptors (TCR) on the surface of T-cells react with
antigen peptide/MHC complexes on the surface of antigen presenting cells
(Specification 2-3).
3
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