Ex Parte Schneck et al - Page 5

                Appeal 2007-1161                                                                                 
                Application 09/954,166                                                                           
                cells and had a high nanomolar affinity for T-cell clones which could be                         
                measured by a direct binding assay (Dal Porto, p. 6671, col. 1 (Abstract);                       
                p. 6672, col. 1; Answer 5).                                                                      
                10.  Dal Porto’s genetically engineered divalent class I MHC molecule                            
                comprises: 1) the extracellular MHC binding (H-2Kb) domain (α1, α2, and                          
                α3) joined to 2) the variable (V) region of IgG heavy chain, forming a                           
                chimeric protein (Dal Porto, p. 6672, cols. 1-2; Fig. 1B).                                       
                11.  To make this molecule, the gene encoding the chimeric protein (H-                           
                2Kb/IgG) was expressed in a cell line (J558L) which also expresses an                            
                immunoglobulin light chain  (Dal Porto, p. 6672, col. 1) and beta2-                              
                microglobulin.                                                                                   
                12.  The resulting molecule was purified from culture.  Analysis showed that                     
                the molecule was divalent (containing two MHC binding sites), consisting                         
                “of dimers of a complex composed of chimeric heavy chain,                                        
                immunoglobulin light chain, and β2-microglobulin (Fig. 1B)” (Dal Porto, p.                       
                6673, col. 2).  The structure of Dal Porto’s molecule is reproduced in the                       
                figure below:                                                                                    








                Dal Porto’s figure shows a protein complex comprising chimeric                                   
                heavy chain, immunoglobulin light chain, and β2-microglobulin.                                   



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