Appeal 2007-1161
Application 09/954,166
13.
Dal Porto et al teach soluble divalent class I MHC/IgG
molecules (H-2Kb/IgG) that demonstrate nanomolar affinities
for T cell receptors and nanomolar concentrations of the soluble
divalent H-2Kb/IgG molecule specifically inhibited lysis of
target cells by . . . T cells, whereas soluble monovalent H-2Kb
never inhibited the response of . . . T cells . . . and previous
indirect measurements of the interaction between soluble
monovalent MHC class I and T cells suggests affinities in the
micromolar range.
(Answer 5). See Dal Porto, p. 6675 (Discussion).
Chang
14. “Generation of soluble T-cell receptor (TCR) molecules by a variety of
genetic engineering methods have been hampered by inefficient pairing of α
and β subunits in the absence of their respective transmembrane regions and
associated CD3 components” (Chang, p. 11408 (Abstract); Answer 5).
15. To overcome this obstacle, Chang adds peptide segments to the
carboxyl termini of the TCR α and β extracellular domains (Chang, p. 11408
(Abstract)).
16. These peptide segments “selectively associate to form a stable
heterodimeric coiled coil termed a leucine zipper” (Chang, p. 11408
(Abstract)).
17. “This approach makes it possible to bring together at will two distinct
subunit components” (Chang, p. 11412, col. 2). See Answer 5.
Harris
18. “Harris et al teaches methods for producing bivalent (i.e., divalent)
binding proteins comprising fusing binding domains via a linker to the N-
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