Appeal 2007-1824
Application 10/639,718
INTRODUCTION
The claims are directed to a method for identifying or evaluating
target compounds (claims 1-17, 43, 44), a method to determine binding
affinity of labeled ligands using microarrays (claim 18), a method for
determining binding affinities between ligands and membrane proteins
(claims 45 and 46), and a method for conducting protein array assays (claims
47-51). Claims 1 and 16 are illustrative:
1. A method for identifying or evaluating target compounds capable
of modulating ligand-receptor interactions, the method comprising: a)
providing a plurality of receptor microspots on a substrate to form an array;
b) preparing a cocktail solution of labeled ligands, each labeled ligand
having an affinity of from about 0.1 nM to about 20 nM and a specificity of
at least 50% to bind with at least one corresponding paired receptor in said
array and a cross activity of no more than 10% with receptors on said array
other than said at least one corresponding paired receptor; c) contacting said
cocktail solution with said array in the presence of a target compound; and
d) detecting a level of binding between each said labeled ligand and its
respective paired receptor, wherein a change in the level of binding between
one of said labeled ligands and its respective paired receptor in the presence
of said target compound, as compared to a control level of binding, is
indicative that said target compound is capable of modulating the interaction
between said one labeled ligand and its respective paired receptor.
16. The method according to claim 1, wherein said method comprises
providing at least two receptors co-immobilized within a single microspot, in
combination with a two-color detection technique, to at least double the
capacity of probe elements.
The Examiner relies on the following prior art references to show
unpatentability:
Bryen A. Jordan, “Opioids and Their Complicated Receptor
Complexes,” Neuropsychopharmacology, 23(54), S5-S18 (2000).
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Last modified: September 9, 2013