Appeal No. 95-4464
Application 07/605,788
20. A method of diagnosing Alzheimer’s disease comprising
the steps of:
i) isolating human tissue containing olfactory neurons;
ii) growing said tissue in a suitable medium under a first
membrane comprising collagen and laminin, establishig a human
olfactory neuron culture;
iii) separating said first membrane from neurons;
iv) replating said neurons on a surface coated with a second
membrane comprising collagen and laminin;
v) culturing said neurons of step (iv) under conditions
allowing replication;
vi) contacting said cultured neurons with a calcium salt;
vii) contacting said neurons of step (vi) with an ionophore;
viii) detecting AD-specific changes in amyloid precursor
protein or A68 as compared to normal; and
ix) diagnosing tissue as AD afflicted, if any AD-specific
changes of said proteins of step (viii) are detected.
The references relied on by the examiner are:
Talamo, et al., (Talamo) “Pathological Changes in Olfactory
Neurons in Patients with Alzheimer’s Disease”, Nature, Vol. 337,
pp. 736-739 (1989).
Coon, et al., (Coon) “Cell Cultures of Neuroblasts from Rat
Olfactory Epithelium that Show Odorant Responses”,Proc. Natl.
Acad. Sci. USA, Vol. 86, pp. 1703-1707 (1989).
Wolozin, et al., (Wolozin) “A Neuronal Antigen in the Brains of
Alzheimer Patients”, Science, Vol. 232, pp. 648-650 (1986).
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