Appeal No. 95-0083
Application 07/711,556
This appeal was taken from the examiner's decision rejecting claims 43, 45
through 49, 52, 54 and 56 through 58, which are the only claims remaining in the
application.
Claims 43 and 54, which are illustrative of the subject matter on appeal, read as
follows:
43. A method for producing a polypeptide of interest in a Kluyveromyces
host cell, said method comprising:
growing a Kluyveromyces host cell comprising an expression
cassette which comprises as components, in the direction of
transcription, a transcriptional regulatory region and translational
initiation region functional in said host cell;
a DNA sequence which encodes said polypeptide of interest;
and
translational and transcriptional termination regions functional in
said host cell, wherein expression of said DNA sequence is under
regulatory control of said transcriptional and translational regions
and wherein said components are operably linked so as to provide
for expression of said DNA sequence, whereby said polypeptide of
interest is expressed in and secreted by said host cell. [emphasis
added]
54. A method for obtaining a polypeptide of interest using a transformed
Kluyveromyces cell, said method comprising:
growing said transformed Kluyveromyces cell comprising a DNA
sequence comprising a region encoding a polypeptide hetero-
logous to said cell, wherein said DNA sequence results from joining
at least two DNA molecules to provide a gene functional for
expression in said Kluyveromyces cell, wherein said gene func-
tional for expression comprises at least a promoter regulation
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