Appeal No. 98-1012 Serial No. 08/117,342 (e.g., E. coli and total coliform bacteria) that one is seeking to detect. For this reason, the examiner concludes Ait would have been obvious to one with ordinary skill in the art at the time Applicant=s invention was made to include the antibiotics and agents to suppress non-coliform gram negative bacteria of Edberg in Manafi=s culture medium@ (second supplemental examiner=s answer, paper no. 48.8, sentence bridging pages 3-4). While we agree that Edberg teaches a medium for simultaneous detection of E. coli and total coliforms (e.g., col. 9, lines 16-21 and claim 16), we are not persuaded that Edberg suggests including an agent to suppress the growth of non-coliform bacteria. The claims clearly require Aat least one agent to 3 suppress growth of non-coliform gram negative bacteria@ and the supporting specification defines this agent as an Ainhibitor@ (page 8, line 11; e.g., antibiotic Cefsulodin 18Page: Previous 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 NextLast modified: November 3, 2007