Appeal No. 98-1012
Serial No. 08/117,342
That passage is directed to the second step of a two-step
process for detecting E. coli in a sample: 1) Gram-negative
bacteria is
segregated from other microbes through the use of yeast-
killing ansiomycin and Gram-positive bacteria-killing
vancomycin, and 2) E. coli is selected from the remaining
Gram-negative bacteria by adjusting the medium to supply a
nutrient-indicator specific to the E. coli. The result of
practicing this example is a medium that encourages the growth
of E. coli at the expense of all other
microbes, including other Gram-negative bacteria: coliforms
and non-coliforms. Since this would defeat the purpose of the
claimed medium B to detect both E. coli and coliforms, the
prima facie case is undermined by this disclosure.
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