Appeal No. 1995-2297
Application No. 07/797,493
4. A chimeric mouse comprising a stable bone marrow graft human
hematopoietic cells, wherein the graft is transplanted by a process comprising:
a. sublethally irradiating an immunodeficient mouse, wherein the
immunodeficient mouse lacks a population of functional T cells and B cells;
6 9
b. infusing from about 10 to about 10 human hematopoietic cells into
the immunodeficient mouse; and
c. administering an effective amount of human mast cell growth factor
(MGF) and a human granulocyte macrophage colony stimulating factor/interleukin-3 fusion
protein (GM-CSF/IL-3 FP) to promote engraftment of the human hematopoietic cells.
9. A process for engrafting human hematopoietic cells in an immunodeficient
mouse comprising:
a. irradiating the immunodeficient mouse with from about 5000 to about
10,000 rads/kg body weight;
6 9
b. infusing from about 10 to about 10 human hematopoietic cells per
kg body weight to the immunodeficient mouse; and
c. administering an effective amount of MGF and GM-CSF/IL-3FP to the
immunodeficient mouse which is effective to promote stable engraftment of human
hematopoietic cells.
13. A chimeric mouse having a stable bone marrow graft of lineage-specific
human hematopoietic cells, wherein the population of lineage-specific human
hematopoietic cells is selected from the group consisting of erythroid cells, myeloid cells,
lymphoid cells and combination thereof, wherein the specific lineage human hematopoietic
cells are obtained by a process comprising:
2
Page: Previous 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Next
Last modified: November 3, 2007